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KMID : 1101420150470040273
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Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 4 p.273 ~ p.278
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Inactivation of Candida albicans Biofilm by Radachlorin-Mediated Photodynamic Therapy
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Kwon Pil-Seung
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Abstract
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The purpose of this study was to evaluate the in-vitro efficacy of PDT using red light emitting diode (LED) with Radachlorin for biofilm inhibition of clinical Candida albicans isolates. The suspensions containing C. albicans at 9×108 CFU/mL were prepared on yeast nitrogen base containing 5% glucose. The biofilm formation was grown for 3 h after seeding suspensions each 100 ul on a 96-well plate and then supernatant was discarded. Each well was treated with 0.39 μg/mL from 50 μg/mL concentrations of Radachlorin on adherent biofilm. After a 30-minute incubation, light was irradiated for 30, 60, or 90 minutes using the following light source of wavelength 630 nm LED, at energy densities of 14, 29, and 43 J/cm2. Afterwards, all supernatant was removed and dried. Adherent cells were stained with safranin O and dried. The cell viability was measured using a microplate reader at 490 nm. Also, a fluorescent signal on C. albicans was observed by saturation of a photosensitizer. In conclusion, a significant inhibition of 72.5% was observed to C. albicans on biofilm at the Radachlorin dose of 50 μg/mL with 630 nm LED. The Photosensitizer (Radachlorin) was adequate at 30 minuttes for C. albicans. Overall, the results showed that inhibition of biofilm formation was Radachlorine dose-dependent. The results suggest that PDT, using Radachlorin with 630 nm LED, is able to decrease biofilm formation of C. albicans.
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KEYWORD
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Biofilm, Candida albicans, Light emmiting diode, Photodynamic therapy, Radachlorin
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